Show simple item record

dc.creatorVähäheikkilä, Marjut
dc.date.accessioned2016-04-20T09:44:53Z
dc.date.available2016-04-20T09:44:53Z
dc.identifier.urihttp://dspace.cc.tut.fi/dpub/handle/123456789/23904
dc.description.abstractLipid domains in plasma membranes are typically nanosized regions with a distinct lipid content. They have been suggested to have multiple functions. For example, the lipid environment can affect the activity of membrane proteins through direct lipid binding or by changing membrane properties. However, the detailed plasma membrane lipid organization and forces driving domain formation have remained elusive, since lipid domains are difficult to study due to their nanoscale size. In this thesis, we study the effects of three different G protein-coupled receptors (GPCRs) on the lateral and transmembrane lipid distributions. To this end, we use self-assembly through molecular dynamics (MD) simulations to study the lipid environment of the β_2-adrenergic receptor, the µ-opioid receptor, and rhodopsin proteins in plasma membrane models that account for the effect of 16 abundant lipid species. Our study reveals that GPCRs affect the lateral organization but not the transmembrane distribution of lipids. Polyunsaturated lipids and one monounsaturated lipid, phosphatidylinositol, are preferred to reside at the protein surface over other studied lipids. Thus, we conclude that GPCR proteins can induce domain formation in plasma membrane models. The results provide important insights into domain formation, which is important to better understand the functioning of GPCR proteins that are essential for human health.en
dc.format.extentXI, 72
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.rightsThis publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.
dc.titleG Protein-Coupled Receptors Induce Domain Formation in Plasma Membrane Models Probed by Molecular Dynamics Simulationsen
dc.title.alternativeG-proteiinikytkentäiset reseptorit saavat aikaan lipidikoostumukseltaan toisistaan poikkeavia solukalvoalueita molekyylidynamiikkasimulaatioiden mukaan
dc.identifier.urnURN:NBN:fi:tty-201604203854
dc.contributor.laitosFysiikan laitos – Department of Physicsen
dc.contributor.laitosKemian ja biotekniikan laitos – Department of Chemistry and Bioengineeringen
dc.contributor.tiedekuntaLuonnontieteiden tiedekunta – Faculty of Natural Sciencesen
dc.contributor.yliopistoTampereen teknillinen yliopisto - Tampere University of Technology
dc.programmeBiotekniikan koulutusohjelmaen
dc.date.published2016-05-04
dc.permissionPermission granteden
dc.contributor.thesisadvisorKulig, Waldemar
dc.contributor.thesisadvisorVattulainen, Ilpo
dc.contributor.degreesupervisorVattulainen, Ilpo
dc.type.ontasotDiplomityö - Master's thesis


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record